|Table of Contents|

Enhancing Nitrogen Removal from Highway Service Area Wastewater by Heterotrophic Nitrification-aerobic Denitrification Bacterium Pseudomonas aeruginosa HC(PDF)

《地球科学与环境学报》[ISSN:1672-6561/CN:61-1423/P]

Issue:
2025年第02期
Page:
248-259
Research Field:
环境与可持续发展
Publishing date:

Info

Title:
Enhancing Nitrogen Removal from Highway Service Area Wastewater by Heterotrophic Nitrification-aerobic Denitrification Bacterium Pseudomonas aeruginosa HC
Author(s):
MA Qing1*;  WU Yan-cheng2;  WU Pei2
(1. Shaanxi Provincial Transport Planning Design and Research Institute Co., Ltd., Xi'an 710065, Shaanxi, China; 2. School of Civil Engineering, Chang'an University, Xi'an 710061, Shaanxi, China)
Keywords:
wastewater treatment;  sequencing batch reactor;  heterotrophic nitrification-aerobic denitrification bacterium;  optimum growth condition;  nitrogen removal;  highway service area
PACS:
X703
DOI:
10.19814/j.jese.2024.10034
Abstract:
Efficient and stable purification of highway service area wastewater is crucial for protecting the surrounding water environment of these service areas. Heterotrophic nitrification-aerobic denitrification(HNAD)bacteria can simultaneously perform nitrification and deni-trification under aerobic conditions, making them valuable in wastewater biological treatment processes. To explore the feasibility of applying the HNAD process to the treatment of highway service area wastewater, a strain with excellent HNAD performance was isolated from a laboratory-scale anaerobic/anoxic/aerobic(A2/O)process reactor. After determining the optimal growth conditions for the strain through batch experiments, the isolated strain were added to a sequencing batch reactor(SBR)process treating highway service area wastewater, and the removal effects of pollutants were inspected. Based on 16s rRNA gene sequencing results, the isolated strain is identified as Pseudomonas aeruginosa HC, belonging to the genus Pseudomonas. The optimal carbon source, C/N ratio, shaker speed, temperature, and pH for the growth of this strain are C4H4Na2O4, 30, 150 rpm, 25 ℃, and 8, respectively. The average effluent concentrations of NH+4-N, NO-2-N, and NO-3-N in the enhanced SBR process are(2.33±0.12),(1.12±0.09)and(8.08±0.11)mg·L-1, respectively. The bioaugmentation of SBR process with strain HC significantly improves the nitrogen removal efficiency, with a total nitrogen(TN)removal efficiency 13.96%±0.25% higher than that of the control reactor. However, after 90 days, the effluent TN of the enhanced SBR process gradually increases, and the bioaugmentation effect begins to diminish. After re-addition of strain HC, the TN removal efficiency of the process gradually recoveres. Maintaining the quantity and activity of HNAD bacteria in the activated sludge system is crucial for the application of the HNAD process in treating wastewater from highway service areas.

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Last Update: 2025-03-20