|本期目录/Table of Contents|

[1]王丽娟,张翼龙,王 哲,等.石油污染土壤生物修复的强化技术[J].地球科学与环境学报,2009,31(04):427-431.
　WANG Li-juan,ZHANG Yi-long,WANG Zhe,et al.Study on Enhanced Technology of Oil-contaminated Soil Bioremediation[J].Journal of Earth Sciences and Environment,2009,31(04):427-431.
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《地球科学与环境学报》[ISSN:1672-6561/CN:61-1423/P]

卷:
第31卷
期数:
2009年第04期
页码:
427-431
栏目:
环境地学
出版日期:
2009-12-15

文章信息/Info

Title:
Study on Enhanced Technology of Oil-contaminated Soil Bioremediation
文章编号:
1672-6561(2009)04-0427-05
作者:
王丽娟; 张翼龙; 王 哲; 侯新伟; 周晓妮
(中国地质科学院 水文地质环境地质研究所,河北 石家庄 050061)
Author(s):
WANG Li-juan;  ZHANG Yi-long;  WANG Zhe;  HOU Xin-wei;  ZHOU Xiao-ni
(Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang 050061, Hebei, China)
关键词:
石油污染; 细菌菌群; 生物强化; 降解
Keywords:
oil-contamination;  bacterial consortium;  bioaugmentation;  degradation
分类号:
X53
DOI:
-
文献标志码:
A
摘要:
为探索石油污染土壤的高效修复方法,从实验室保存的优势菌中筛选得到4株降油效果最佳菌,采用摇床和恒温培养箱培养,对含油量为5%的石油污染土壤进行微生物菌剂强化处理和环境强化实验。微生物菌剂强化结果表明:4种菌和除油效果最好的A、C、D混合菌3d可将石油烃依次降解24%、19.81%、22.55%、26.46%、39.67%;并对该菌群的最佳投加配比进行确定,A、C、D菌群数量的最佳配比为NA:NC:ND=1:2:0.5,3d内菌群A、C、D在最佳接种配比情况下可将石油烃降解44.2%。环境强化实验结果表明:A、C、D菌群在最佳修复条件营养物质C:N:P为75:8:3、表面活性剂为0.5%、通气条件为6层纱布、电子受体H2O2的加入量为1.5%下,3d内石油烃降解61.46%,比自然条件下修复的除油率4.7%提高了56.76%,较只进行菌种强化时最高除油率44.2%提高了约17%。
Abstract:
In order to explore better treatment process of oil-contaminated soil,four optimized bacteria that can degrade petroleum efficiently are selected from the predominant bacteria which are stored by our laboratory. Their environmental strengthening test and microbial agent strengthening processing were conducted using shaking cultivation and incubator in the oil-contaminated soil containing 5% oil. The results of microbial agent strengthening indicate that the reduction of petroleum hydrocarbons of four strains and a mixed strain are 24%, 19.81%, 22.55%, 26.46% and 39.67% respectively. A test performed to determine the best ratio of the flora ACD shows that the best number ratio of the flora A, C and D is NA:NC:ND=1:2:0.5 within 3 d, and the flora A, C and D in the best circumstances can degrade 44.2% oil. The results of environmental strengthening test indicate that the flora A, C and D in the best conditions for the ratio of nutrients C:N:P is 75:8:3, and the content of surfactant is 0.5%. In the ventilation conditions for the six gauze and E-receptor H2O2 is 1.5%, the degradation of petroleum hydrocarbons is 61.46% within 3 d, and the degradation rate increase 56.76% compared with that under natural conditions which is only 4.7%, and increase 17% compared with the degradation rate of 44.2% when only strengthening the bacteria.

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相似文献/References:

[1]李春荣,王文科,曹玉清,等.石油污染土壤对向日葵生长的影响[J].地球科学与环境学报,2006,28(04):97.
　LI Chun-rong,WANG Wen-ke,CAO Yu-qing,et al.Influences of Petroleum-contaminated Soil on Growth of Sunflower LI Chun-rong1，WANG Wen-ke1，CAO Yu-qing 2，WANG Li-juan1[J].Journal of Earth Sciences and Environment,2006,28(04):97.

备注/Memo

备注/Memo:
收稿日期:2009-01-20
基金项目: 国家重点基础研究发展计划项目(G1999043606)
作者简介: 王丽娟(1981-),女,吉林长春人,实习研究员,从事地下水开发利用研究。E-mail: wanglijuan0486@163.com

更新日期/Last Update: 2009-12-20