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《地球科学与环境学报》[ISSN:1672-6561/CN:61-1423/P]

卷:

第47卷

期数:

2025年第02期

页码:

248-259

栏目:

环境与可持续发展

出版日期:

2025-03-15

文章信息/Info

Title:

Enhancing Nitrogen Removal from Highway Service Area Wastewater by Heterotrophic Nitrification-aerobic Denitrification Bacterium Pseudomonas aeruginosa HC

文章编号:

1672-6561(2025)02-0248-12

作者:

马 青^1*; 吴彦成²; 吴 沛²

(1. 陕西省交通规划设计研究院有限公司,陕西 西安 710065; 2. 长安大学 建筑工程学院,陕西 西安 710061)

Author(s):

MA Qing^1*;  WU Yan-cheng²;  WU Pei²

(1. Shaanxi Provincial Transport Planning Design and Research Institute Co., Ltd., Xi'an 710065, Shaanxi, China; 2. School of Civil Engineering, Chang'an University, Xi'an 710061, Shaanxi, China)

关键词:

污水处理;  序批式反应器;  异养硝化-好氧反硝化菌;  最佳生长条件;  脱氮;  高速公路服务区

Keywords:

wastewater treatment;  sequencing batch reactor;  heterotrophic nitrification-aerobic denitrification bacterium;  optimum growth condition;  nitrogen removal;  highway service area

分类号:

X703

DOI:

10.19814/j.jese.2024.10034

文献标志码:

A

摘要:

实现高速公路服务区污水的高效、稳定净化,对服务区周边水环境的保护有重要意义。异养硝化-好氧反硝化(HNAD)菌可以在好氧条件下同时实现硝化和反硝化,在污水生物处理过程中具有应用价值。为探究异养硝化-好氧反硝化过程应用于高速公路服务区污水处理的可行性,从实验室规模的厌氧/缺氧/好氧(A²/O)反应器中分离得到一株具有良好异养硝化-好氧反硝化性能的菌株,在采用批次实验探明该菌株最佳生长条件的基础上,将该菌株加入处理高速公路服务区污水的序批式反应器(SBR),探究了SBR工艺对污染物的去除效果。根据16s rRNA基因测序结果,发现分离得到的菌株为Pseudomonas aeruginosa HC,属于假单胞菌属。该菌株生长的最佳碳源为C₄H₄Na₂O₄,最佳碳氮比(C/N)为30,最佳摇床转速为150 rpm,最佳温度为25 ℃,最佳pH值为8。强化SBR工艺出水的NH⁺₄-N、NO^-₂-N和NO^-₃-N平均浓度分别为(2.33±0.12)、(1.12±0.09)和(8.08±0.11)mg·L^-1。与控制反应器相比,强化SBR工艺的生物强化脱氮效果提升明显,总氮(TN)去除率比控制反应器高13.96%±0.25%。90 d后,强化SBR工艺出水总氮逐渐升高,生物强化效果开始减弱; 再次加入HC菌后,工艺的总氮去除率(TNRE)逐步恢复。

Abstract:

Efficient and stable purification of highway service area wastewater is crucial for protecting the surrounding water environment of these service areas. Heterotrophic nitrification-aerobic denitrification(HNAD)bacteria can simultaneously perform nitrification and deni-trification under aerobic conditions, making them valuable in wastewater biological treatment processes. To explore the feasibility of applying the HNAD process to the treatment of highway service area wastewater, a strain with excellent HNAD performance was isolated from a laboratory-scale anaerobic/anoxic/aerobic(A²/O)process reactor. After determining the optimal growth conditions for the strain through batch experiments, the isolated strain were added to a sequencing batch reactor(SBR)process treating highway service area wastewater, and the removal effects of pollutants were inspected. Based on 16s rRNA gene sequencing results, the isolated strain is identified as Pseudomonas aeruginosa HC, belonging to the genus Pseudomonas. The optimal carbon source, C/N ratio, shaker speed, temperature, and pH for the growth of this strain are C₄H₄Na₂O₄, 30, 150 rpm, 25 ℃, and 8, respectively. The average effluent concentrations of NH⁺₄-N, NO^-₂-N, and NO^-₃-N in the enhanced SBR process are(2.33±0.12),(1.12±0.09)and(8.08±0.11)mg·L^-1, respectively. The bioaugmentation of SBR process with strain HC significantly improves the nitrogen removal efficiency, with a total nitrogen(TN)removal efficiency 13.96%±0.25% higher than that of the control reactor. However, after 90 days, the effluent TN of the enhanced SBR process gradually increases, and the bioaugmentation effect begins to diminish. After re-addition of strain HC, the TN removal efficiency of the process gradually recoveres. Maintaining the quantity and activity of HNAD bacteria in the activated sludge system is crucial for the application of the HNAD process in treating wastewater from highway service areas.

参考文献/References:

[1] JTG B01—2014,公路工程技术标准[S].
JTG B01—2014,Technical Standard of Highway Engineering[S].
[2] 薛鹏丽,孙晓峰,宋 云,等.高速公路服务区污水回用水质标准研究[J].公路交通科技(应用技术版),2013,9(4):119-122.
XUE Peng-li,SUN Xiao-feng,SONG Yun,et al.Stu-dy on Water Quality Standards for Sewage Reuse in Expressway Service Areas[J].Highway Transportation Technology(Applied Technology Edition),2013,9(4):119-122.
[3] 陈 瑶,黄学文,孔祥燕,等.服务区污水处理设施长期运行效果及菌群特性[J].交通运输研究,2022,8(4):137-144.
CHEN Yao,HUANG Xue-wen,KONG Xiang-yan,et al.Long-term Operation Effect and Bacterial Community Characteristics of Sewage Treatment Facilities in the Service Area[J].Transport Research,2022,8(4):137-144.
[4] 林 奇.高速公路服务区污水特性研究[J].能源与环境,2013(1):12-14,17.
LIN Qi.Study on the Characteristics of Sewage in Expressway Service Areas[J].Energy and Environment,2013(1):12-14,17.
[5] 杨志敏,陈玉成,何 娟,等.我国高速公路服务区污水水质的集对分析[J].三峡环境与生态,2010,32(5):6-10.
YANG Zhi-min,CHEN Yu-cheng,HE Juan,et al.Set Pair Analysis on the Quality of Sewage from Highway Service Areas in China[J].Environment and Eco-logy in the Three Gorges,2010,32(5):6-10.
[6] 梁丽萍,张 林.江苏省高速公路服务区污水处理现状及解决对策[J].中国给水排水,2017,33(4):20-25.
LIANG Li-ping,ZHANG Lin.Problems and Solutions for Sewage Treatment in Expressway Service Areas in Jiangsu Province[J].China Water & Was-tewater,2017,33(4):20-25.
[7] T/CCTAS XX—2024,公路服务与管理区污水处理技术指南(征求意见稿)[S].
T/CCTAS XX—2024,Technical Guidelines for Se-wage Treatment of Highway Service and Management Area(Draft for Comment)[S].
[8] 吴 沛,朱继涛,顾 鑫,等.多段进水A/O生物接触氧化工艺模拟[J].地球科学与环境学报,2021,43(5):908-916.
WU Pei,ZHU Ji-tao,GU Xin,et al.Simulation of Step-feeding A/O Biological Contact Oxidation Pro-cess[J].Journal of Earth Sciences and Environment,2021,43(5):908-916.
[9] 马兴冠,宁 宇,李洪波.高速公路服务区集中式污水处理工艺及综合评价[J].沈阳建筑大学学报(自然科学版),2020,36(5):947-954.
MA Xing-guan,NING Yu,LI Hong-bo.Comprehensive Evaluation of Centralized Sewage Treatment Te-chnology in Expressway Service Area[J].Journal of Shenyang Jianzhu University(Natural Science),2020,36(5):947-954.
[10] 岳志轩,夏阳光,杨小丽,等.典型高速公路服务区污水水质与处理效果调研分析[J].公路交通科技,2024,41(11):199-206.
YUE Zhi-xuan,XIA Yang-guang,YANG Xiao-li,et al.Investigation and Analysis on Sewage Quality and Treatment Effect in Typical Expressway Service Areas[J].Journal of Highway and Transportation Research and Development,2024,41(11):199-206.
[11] 徐 亮,陈 鹏,成 波,等.MABR技术在高速公路服务区污水处理中的应用[J].净水技术,2022,41(9):72-78.
XU Liang,CHEN Peng,CHENG Bo,et al.Application of MABR Technology in Wastewater Treatment of Expressway Service Area[J].Water Purification Technology,2022,41(9):72-78.
[12] 谭丰佚,刘新颖,党 岩,等.有机碳源对异养硝化-好氧反硝化生物脱氮的影响及其优化[J].环境工程学报,2024,18(4):1183-1191.
TAN Feng-yi,LIU Xin-ying,DANG Yan,et al.Effect of Organic Carbon on the Biological Nitrogen Remo-val Through Heterotrophic Nitrification-aerobic Denitrification and Its Optimization[J].Chinese Journal of Environmental Engineering,2024,18(4):1183-1191.
[13] CHEN J,GU S Y,HAO H H,et al.Characteristics and Metabolic Pathway of Alcaligenes sp.TB for Simultaneous Heterotrophic Nitrification-aerobic De-nitrification[J].Applied Microbiology and Biotechno-logy,2016,100(22):9787-9794.
[14] ROBERTSON L A,KUENEN J G.Aerobic Denitrification:A Controversy Revived[J].Archives of Microbiology,1984,139(4):351-354.
[15] GU X,LENG J T,ZHU J T,et al.Influence Mechanism of C/N Ratio on Heterotrophic Nitrification-aerobic Denitrification Process[J].Bioresource Technology,2022,343:126116.
[16] LENG J T,LU J Y,HAI C,et al.Exploring Influence Mechanism of Small-molecule Carbon Source on He-terotrophic Nitrification-aerobic Denitrification Pro-cess from Carbon Metabolism,Nitrogen Metabolism and Electron Transport Process[J].Bioresource Te-chnology,2023,387:129681.
[17] LI S S,HE Z M,LI C,et al.Nitrogen Removal by He-terotrophic Nitrification-aerobic Denitrification Bacteria:A Review[J].Desalination and Water Treatment,2024,317:100277.
[18] XI H P,ZHOU X T,ARSLAN M,et al.Heterotro-phic Nitrification and Aerobic Denitrification Process:Promising but a Long Way to Go in the Wastewater Treatment[J].Science of the Total Environment,2022,805:150212.
[19] 杨 婷,杨 娅,刘玉香.异养硝化-好氧反硝化的研究进展[J].微生物学通报,2017,44(9):2213-2222.
YANG Ting,YANG Ya,LIU Yu-xiang.Research Progress and Challenges of Heterotrophic Nitrification-aerobic Denitrification[J].Microbiology China,2017,44(9):2213-2222.
[20] ZHANG Q,CHEN X,ZHANG Z Y,et al.Perfor-mance and Microbial Ecology of a Novel Moving Bed Biofilm Reactor Process Inoculated with Heterotrop-hic Nitrification-aerobic Denitrification Bacteria for High Ammonia Nitrogen Wastewater Treatment[J].Bioresource Technology,2020,315:123813.
[21] YANG L,REN Y X,LIANG X,et al.Nitrogen Removal Characteristics of a Heterotrophic Nitrifier Acinetobacter Junii YB and Its Potential Application for the Treatment of High-strength Nitrogenous Was-tewater[J].Bioresource Technology,2015,193:227-233.
[22] XIANG Z Z,CHEN X,BAI J,et al.Salt-tolerant He-terotrophic Nitrification and Aerobic Denitrification(HNAD)Bacterium Enhancing a Pilot-scale Moving Bed Biofilm Reactor(MBBR)Treating Mariculture Wastewater Treatment with Different COD/TN Ratios[J].Journal of Water Process Engineering,2022,50:103278.
[23] SONG K N,GAO Y W,YANG J,et al.Performance of Simultaneous Carbon and Nitrogen Removal of High-salinity Wastewater in Heterotrophic Nitrification-aerobic Denitrification Mode[J].Journal of Environmental Chemical Engineering,2023,11(3):109-682.
[24] HEMALATHA M,SRAVAN J S,YERUVA D K,et al.Integrated Ecotechnology Approach Towards Treatment of Complex Wastewater with Simultaneous Bioenergy Production[J].Bioresource Technology,2017,242:60-67.
[25] XIA L,LI X M,FAN W H,et al.Heterotrophic Nitrification and Aerobic Denitrification by a Novel Acinetobacter sp.ND7 Isolated from Municipal Activated Sludge[J].Bioresource Technology,2020,301:122749.
[26] YAO Z B,YANG L,WANG F,et al.Enhanced Nitrate Removal from Surface Water in a Denitrifying Woodchip Bioreactor with a Heterotrophic Nitrifying and Aerobic Denitrifying Fungus[J].Bioresource Te-chnology,2020,303:122948.
[27] 国家环境保护总局.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
State Environmental Protection Administration.Monitoring and Analysis Method of Water and Waste-water[M].4th ed.Beijing:China Environmental Sciences Press,2002.
[28] HUANG M Q,CUI Y W,HUANG J L,et al.A Novel Pseudomonas Aeruginosa Strain Performs Simultaneous Heterotrophic Nitrification-aerobic Denitrification and Aerobic Phosphate Removal[J].Water Research,2022,221:118823.
[29] HU B,LU J Y,QIN Y X,et al.A Critical Review of Heterotrophic Nitrification and Aerobic Denitrification Process:Influencing Factors and Mechanisms[J].Journal of Water Process Engineering,2023,54:103995.
[30] SILVA L C F,LIMA H S,MENDES T A O,et al.Heterotrophic Nitrifying/Aerobic Denitrifying Bacteria:Ammonium Removal Under Different Physical-chemical Conditions and Molecular Characterization[J].Journal of Environmental Management,2019,248:109294.
[31] ZHANG H H,LI S L,MA B,et al.Nitrate Removal Characteristics and ¹³C Metabolic Pathways of Aerobic Denitrifying Bacterium Paracoccus Denitrificans Z195[J].Bioresource Technology,2020,307:123230.
[32] CHEN L F,CHEN L X,PAN D,et al.Heterotrophic Nitrification and Related Functional Gene Expression Characteristics of Alcaligenes Faecalis SDU20 with the Potential Use in Swine Wastewater Treatment[J].Bioprocess and Biosystems Engineering,2021,44(10):2035-2050.
[33] 梁 贤,任勇翔,杨 垒,等.异养硝化-好氧反硝化菌YL的脱氮特性[J].环境科学,2015,36(5):1749-1755.
LIANG Xian,REN Yong-xiang,YANG Lei,et al.Characteristics of Nitrogen Removal by a Heterotro-phic Nitrification-aerobic Denitrification Bacterium YL[J].Environmental Science,2015,36(5):1749-1755.
[34] 杨荣武.生物化学原理[M].2版.北京:高等教育出版社,2012.
YANG Rong-wu.Principles of Biochemistry[M].2nd ed.Beijing:Higher Education Press,2012.
[35] DONG Y H,WANG Z Y,LI L,et al.Heterotrophic Nitrification and Aerobic Denitrification Characteristics of the Psychrotolerant Pseudomonas Peli NR-5 at Low Temperatures[J].Bioprocess and Biosystems Engineering,2023,46:693-706.
[36] KE X,LIU C,YU H,et al.Coculture of the Heterotrophic Nitrification-aerobic Denitrification Strains Acinetobacter sp.A12 and Paracoccus sp.T8 Enables Effective Biological Nitrogen Removal from Landfill Leachate[J].ACS Environmental Science & Techno-logy Water,2024,4(4):1786-1797.
[37] ZHAO B,CHENG D Y,TAN P,et al.Characterization of an Aerobic Denitrifier Pseudomonas Stutzeri Strain XL-2 to Achieve Efficient Nitrate Removal[J].Bioresource Technology,2018,250:564-573.
[38] CHEN S H,HE S Y,WU C J,et al.Characteristics of Heterotrophic Nitrification and Aerobic Denitrification Bacterium Acinetobacter sp.T1 and Its Application for Pig Farm Wastewater Treatment[J].Journal of Bioscience and Bioengineering,2019,127(2):201-205.
[39] RAJTA A,BHATIA R,SETIA H,et al.Role of He-terotrophic Aerobic Denitrifying Bacteria in Nitrate Removal from Wastewater[J].Journal of Applied Microbiology,2020,128(5):1261-1278.
[40] 冯 亮,袁春燕,杨 超,等.好氧反硝化生物脱氮技术的研究进展[J].微生物学通报,2020,47(10):3342-3354.
FENG Liang,YUAN Chun-yan,YANG Chao,et al.Research Progress in Nitrogen Removal by Aerobic Denitrification[J].Microbiology China,2020,47(10):3342-3354.
[41] LI K,XIA J,MEHMOOD M A,et al.Extracellular Redox Potential Regulation Improves Yeast Tolerance to Furfural[J].Chemical Engineering Science,2019,196:54-63.
[42] RUAN Y J,TAHERZADEH M J,KONG D D,et al.Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Ha-lotolerant Pseudomonas Balearica Strain RAD-17[J].Microorganisms,2020,8(1):72.
[43] FARRELL J,ROSE A.Temperature Effects on Microorganisms[J].Annual Review of Microbiology,1967,21:101-120.
[44] YANG M,LU D W,QIN B D,et al.Highly Efficient Nitrogen Removal of a Coldness-resistant and Low Nutrient Needed Bacterium,Janthinobacterium sp.M-11[J].Bioresource Technology,2018,256:366-373.
[45] DUAN J M,FANG H D,SU B,et al.Characterization of a Halophilic Heterotrophic Nitrification-aerobic Denitrification Bacterium and Its Application on Treatment of Saline Wastewater[J].Bioresource Technology,2015,179:421-428.
[46] YANG J R,WANG Y,CHEN H,et al.Ammonium Removal Characteristics of an Acid-resistant Bacterium Acinetobacter sp.JR1 from Pharmaceutical Was-tewater Capable of Heterotrophic Nitrification-aerobic Denitrification[J].Bioresource Technology,2019,274:56-64.
[47] GB 18918—2002,城镇污水处理厂污染物排放标准[S].
GB 18918—2002,Discharge Standard of Pollutants from Urban Sewage Treatment Plants[S].
[48] GB 8978—1996,污水综合排放标准[S].
GB 8978—1996,Integrated Wastewater Discharge Standard[S].
[49] 简 丽.我国公路服务区污水处理现状问题及技术对策[J].公路交通科技(应用技术版),2018,14(8):319-322.
JIAN Li.Current Status,Problems,and Technical Countermeasures of Sewage Treatment in Highway Service Areas in China[J].Highway Transportation Science and Technology(Applied Technology Edition),2018,14(8):319-322.
[50] WANG J L,CHEN P Z,LI S P,et al.Mutagenesis of High-efficiency Heterotrophic Nitrifying-aerobic Denitrifying Bacterium Rhodococcus sp.Strain CPZ 24[J].Bioresource Technology,2022,361:127692.
[51] JIA Y T,ZHOU M M,CHEN Y C,et al.Insight into Short-cut of Simultaneous Nitrification and Denitrification Process in Moving Bed Biofilm Reactor:Effects of Carbon to Nitrogen Ratio[J].Chemical Engineering Journal,2020,400:125905.
[52] ADAV S S,CHEN M Y,LEE D J,et al.Degradation of Phenol by Aerobic Granules and Isolated Yeast Candida Tropicalis[J].Biotechnology and Bioengineering,2007,96(5):844-852.
[53] 王亚军,蔡文娟,王 惠.生物强化技术及其在污水处理中应用研究评述[J].生态环境学报,2020,29(5):1062-1070.
WANG Ya-jun,CAI Wen-juan,WANG Hui.Bioaugmentation Technology and Its Application in Waste-water Treatment[J].Ecology and Environmental Sciences,2020,29(5):1062-1070.
[54] MA H L,ZHAO Y X,YANG K C,et al.Application Oriented Bioaugmentation Processes:Mechanism,Per-formance Improvement and Scale-up[J].Bioresource Technology,2022,344:126192.
[55] LOU L,LUO H Y,FANG J,et al.The Advance of Heterotrophic Nitrification Aerobic Denitrification Microorganisms in Wastewater Treatment[J].Bioresour-ce Technology Reports,2023,22:101495.

相似文献/References:

[1]杨文娟.神经网络和主元分析—神经网络软测量技术 在污水处理系统中的应用[J].地球科学与环境学报,2008,30(01):101.
　YANG Wen-juan.Soft Sensors with ANN and PCA-ANN for Wastewater Treatment System[J].Journal of Earth Sciences and Environment,2008,30(02):101.

备注/Memo

备注/Memo:

收稿日期:2024-10-24; 修回日期:2025-02-18
基金项目:陕西省交通运输厅2023年度科研项目(23-76K)
*通信作者:马 青(1984-),女,陕西榆林人,正高级工程师,工学博士,E-mail:3972316431@qq.com。
通信作者:吴 沛(1983-),男,陕西西安人,高级工程师,E-mail:peterwoody@chd.edu.cn。

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更新日期/Last Update: 2025-03-20